The distribution coefficient (k) values were used to estimate the affinity of NIPs to glabridin.
We estimate the affinity (KD) of the polymeric site for nucleotide triphosphates to be approximately 10 -4 M.
A single-point Kd calculation method established earlier for binding evaluation of pure ligands or simple ligand mixtures was then employed to estimate the affinity of each ligand to the receptor (Qin et al. 2015).
The aforementioned MD-data ("randX", where X = M1, M2, L3 and P1) were used to estimate the affinity of hPS to the related sites on the protein surface.
As a consequence, it is difficult to estimate the affinity of AMPARs for scaffold elements, or the density and distribution of scaffold proteins in the PSD.
Because of the spectroscopically quiet nature of Zn II), a metallochromic indicator, zincon, was used to estimate the affinity of Zn II) for YjiA.
Titration experiments led us to estimate the affinity of CF1 for the complex to be low (3.1 mM ± 1.3; Figure 3C), as expected for a small chemical fragment (168 Da).
QDAT software (ICX Nomadics) was used to analyze the sensorgrams for the kinetics of binding (ka) and dissociation (kd) and the SPR binding curves to estimate the affinity of binding (Kd).
Orthovanadate binds to the active sites of numerous enzymes including phosphatases and kinases.[ 24] To estimate the affinity of orthovanadate for PTEN ox), we performed V NMR experiments with preoxidized PTEN.
In order to get a measurement for genetic distance and thus to estimate the affinity between the diverse tumor entities, we applied the Cohen's Kappa Coefficient [ 21] to our binarized functional cancer map and clustered the distant relation matrix (figure 3).
However, these titrations show that addition of U2A′ significantly increases the affinity of U1A for SLIV, and fitting these data to our binding model allowed us to estimate the affinity of U2A′ for U1A [ KU2A′,app = (1.4 ± 0.2) × 10 6 M].
