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To illustrate their applicability, we used GAP models to estimate richness of all native, terrestrial vertebrates within Arizona and for those vertebrates grouped by class or by sensitivity to the type of facility infrastructure.
The freeware program EstimateS version 8.2 [51] was used to compute accumulation curves and estimate richness of naturally-occurring arrangement types.
These detail the number of observed OTU, estimate the number of observed and unobserved OTU, and estimate richness weighted by phylogenetic differences such that related OTU contribute less to the statistic than unrelated OTU, respectively.
Fourteen was selected due to being the maximum number of sites available for two of the strategies, and nine sites (total for time-based) was recognized as being too few sites to accurately estimate richness for several of the strategies.
While these analyses showed that none of the employed sampling strategies captured the total richness present in the system, there was variability among the strategies in their ability to estimate richness.
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When the decline of zeta diversity follows the negative exponential form, the zeta-based richness estimator becomes the same as the incidence-based Chao II estimator which estimates richness based on the frequency of singletons and doubletons in samples.
Both the Chao 1 and the ACE estimators pinpointed SNC as the habitat with the highest estimated richness values (1,240 and 2,115, respectively; Table 2).
At 3 and 5% genetic distance, the rarefaction curves were not saturated and the richness estimators indicated that 35.5 to 89.3% and 38.9 to 84.8% of the estimated richness, respectively, were recovered by the sequencing effort (Figures 1, 2 and S1, and Table S3).
Estimated richness of bacterial and fungal species of KMC is shown in Table 1.
Obviously, the uncertainty in estimating richness will increase with decreasing contiguous plot area.
and an estimated richness S2 for a plot with area2 {S}_2=Aast {lexp(1- exp left -mathrm{b}ast {mathrm{area}}_2right)right)}^{mathrm{c}} (3).
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