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Instead, an estimate of sequence identity is obtained at a price and effort far below that of whole genome sequencing.
It is hypothesized that a conservative estimate of sequence errors in bacterial sequences deposited to GenBank today is less than 0.1%.
It is notable also that the lower bound estimate of sequence constraint is far in excess of the quantity of protein-coding sequence (approximately 29 Mb) in avian genomes, implying that the majority of functional sequence in avian genomes is noncoding, probably regulatory, sequence.
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Interestingly, none of the pathways with either elevated or diminished pathway level transcript CV showed significantly different estimates of sequence polymorphism in comparison to randomly generated pathways (Table S5)[52].
This would produce upwardly biased estimates of sequence divergence.
We excluded these substitutions from our estimates of sequence divergence between ancestral and descendant repeat element copies, producing refined estimates of sequence divergence.
This would produce upwardly biased estimates of sequence divergence from consensus.
Estimates of sequence diversity are highest in the Yukon River and Kuskokwim River drainages near the Bering Sea.
These suboptimal alignments may lead to less accurate estimates of sequence divergence.
However, the availability of protein domains and their corresponding nucleotide sequences linked to estimates of sequence evolutionary rates is lacking.
We also constructed Neighbor-joining trees [ 39] of the amino acid sequences using MEGA4 [ 40] and Poisson-corrected estimates of sequence divergence.
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