Moreover, most of our estimates of duplication time were less than the species divergence time reported by Tamura [ 38].
If the cancer trait has a larger heritability, then a sample size of 400 will provide good estimates of duplication rates.
Another explanation is that the available full genome sequences do not allow for appropriate estimates of duplication rates in these clades.
It is thus unlikely that their inclusion altered the pooled risk estimate because of duplication.
Because the probability of multiple duplication events affecting a single gene increases, the pairwise estimate of the duplication rate becomes increasingly inaccurate beyond dS = 0.3.
Thus, to obtain a more accurate estimate of the duplication time, it is necessary to use a larger data set.
This method remains an estimate of library duplication due to the possibility of two independent molecules carrying the same UMI and UMI sequence alteration during subsequent amplification.
Comparing Guthrie records with GROS provides an estimate of 1.6% duplication of Guthrie data at 5-7 days, owing to repeat tests.
We derived an estimate of the duplication content of the bovine genome to be 3.11% (94.4 Mb/3,036.6 Mb; Fig. 2 and also see Additional File 1: Fig. S1).
