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The estimate of diversity in the communities suggests that the original field soil harbored the most diverse communities, and all soil treatments reduced the diversity.
The inverse of the Simpson dominance (d s ) was preferred to the Simpson diversity index (D s ) for the analysis given that the latter provides a good estimate of diversity at relatively small sample sizes (Magurran 2004).
Despite the lack of data, we have herein created a baseline estimate of diversity for the region, which will aid in the monitoring and detection of future changes occurring in the system in future.
Through an extensive survey of existing studies, databases, museum records, and unpublished information (where possible), we present the first estimate of diversity for the deep-sea Gulf of Maine region: 1671 species, distributed across 2 Kingdoms and 18 Phyla.
Developing a baseline estimate of diversity is absolutely imperative, particularly in light of the future potential anthropogenic impacts in the deep-sea Gulf of Maine region: offshore waste disposal, chemical contamination, expanding oil and gas exploration, alien species, increased ship traffic, fishing to progressively deeper waters, and climate change [2], [85].
The highest discrepancy rate (454 to Illumina: 0.0071%) was an order of magnitude below the overall estimate of diversity obtained for the sperm whale mitogenome (π = 0.096%).
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Systematic biases in estimates of diversity were created by removing unresolved nuclear alleles from the dataset [67].
In order to generate accurate estimates of diversity, our sampling scheme consisted of a replicated sampling design and large area of coverage of the stations [21].
Of course, these estimates of diversity may be biased by the coverage of the primers and amplification conditions, and key lineages could possibly remain undetected [22].
The estimates of diversity, richness, evenness and library coverage for the three 16S rDNA clone libraries studied are shown in Table 4.
Methods of sampling differ by target species or taxonomic group, as well as substrate type, yielding different estimates of diversity or abundance for different taxonomic groups within and among habitats (e.g. [28], [42]).
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