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Effectively using the Resc sample as a biological replicate for the control group will lead to a potential over-estimation of biological variation resulting in a conservative estimate of differential H3K4me3 patterns.
The error of the estimate of differential outcome of treatment and control interventions was consistently lower using the proposed pair-wise compatibility-based method than the baseline repeated random-draws algorithm.
After linear model fitting, a Bayesian estimate of differential expression was calculated and the threshold for statistical significance was set at p<0.005 (Bayesian modified t-test).
After linear model fitting, a Bayesian estimate of differential expression was calculated.
This procedure commonly yields dispersion estimates that are higher than with replicated treatments, resulting in a more conservative estimate of differential expression.
The variance-covariance matrix had a diagonal structure with off-diagonals of zero, and diagonals representing the variance of the estimate of differential expression within study.
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A realistic ionospheric model is essential for accurate ray tracing results and for estimates of differential error that are accurate on sub-centimetre scales.
Estimates of differential expression were adjusted for multiple testing using the false discovery rate method [ 69].
Consequently, estimates of differential expression presented here must be treated cautiously.
All estimates of differential expression were adjusted for multiple testing using the false discovery rate method [ 46].
Although widely used, there are reports that the RPKM method can bias estimates of differential expression [ 40, 43].
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