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In the case of the de novo sequencing projects the genome size can be judged from estimate of coverage (usually modeled as a dispersed Poisson) from a subsample of contigs (with some care for eliminating the outliers coming from repeats or difficult to sequence regions).
Confidence intervals of 95% have an average width of ±6% around the point estimate of coverage.
A second estimate of coverage of the target genomes by aligned contigs was obtained by adding up the distances between anchor marker BLAST matches and excluding overlaps.
A crude estimate of coverage often used is the number of vaccine doses distributed divided by the size of the target population.
From this estimate of coverage we can also calculate the number of additional reads required to discover a new gene: CAP3 [ 41] was used to perform assembly of the processed ESTs.
The other two strategies enable analyses of repetitive regions, with the best match approach providing a reasonable estimate of coverage [ 12] and reporting all possible alignments to avoid erroneous choices about read placement.
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Moreover, he said, if the law is repealed, 32 million fewer people will have health insurance in 2019, compared with estimates of coverage under the existing law.
The lowest estimates of coverage were for winter and night-time samples.
However, as we did not find any child without parents or legal guardians during enrolment of study subjects in the district, the issue of an over-estimate of coverage did not arise.
Because of the cross-sectional nature of the study, possible misclassification of some toddlers who were vaccinated but did not possess documentary evidence as "non-vaccinated" resulting in an under-estimate of coverage is another limitation.
This increases the uncertainty in our estimates of coverage parameters.
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