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Indeed, although the slope of a standard curve provides an estimate of amplification efficiency, a similar assumption must still be made; that is that the amplification efficiencies of all samples are identical, or at least similar, to that predicted by a standard curve.
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The estimates of amplification efficiencies were calculated for each of the 12 genes using the formula Efficiency = 2 − 1 / slope − 1 where slope was estimated from the regression of CT measurements versus RNA concentration.
We made conservative estimates of amplification factors, knowing that they are prone to sampling errors in the actual number of initial cells in each sample, in addition to variable efficiency in isolation of total RNA.
Estimates of amplification efficiencies were obtained for all gene assays and are provided by the formula: Efficiency = 100 × 2 − 1 / slope − 1 where the slope is estimated from the regression of Cp measurements versus (known) log base 2 RNA concentration described above.
An accurate estimate of dynamic amplification for bridges is demonstrated with a wide range of good profiles.
The degree to which other differences between the models can be sufficiently minimized to prevent them from biasing the quantitative estimate of confounding amplification also deserves investigation.
The estimated age of amplification of the Helibats and the rapid radiation of vesper bats are roughly coincidental and suggest that the invasion and amplification of these elements might have influenced their evolutionary trajectory potentially contributing to phenotypic and genotypic diversity.
Part of the problem — for me anyway — concerns amplification.
To more flexibly estimate the relationship of amplification success to sample age, we fit a binomial general additive linear model (GAM) to the success data with age as a covariate.
The most reliable estimates of site amplification are obtained by analyzing the recorded motions of the site during strong earthquakes.
Figure 2 shows the station distribution of (a) successfully estimated and (b) not estimated of site amplification factors and site-correct filters.
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