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Here we present sequence data for the mitochondrial genes cytochrome b (Cytb) and 16 s and a nuclear recombination activating gene-1 (Rag1) for Amphiuma from across the geographic distribution of the family to estimate lineage diversity.
To estimate lineage diversity of Amphiuma we amplified portions of two mitochondrial genes, Cytb (783 bp) and 16 s (538 bp), and the nuclear gene Rag1 (825 bp) from specimens from across the distribution of the three species (Figure 1; Table S1).
We utilized molecular phylogenetic approaches and secondary calibrations points to estimate lineage ages.
A coalescent-based isolation-with-migration (IM) model was used to estimate lineage divergence times and population demographic parameters.
We estimate lineage divergence times within the group and attempt to reconstruct events in its biogeographic history.
Finally, we train LineageProgram on the full dataset to estimate lineage programs and use cross-validation on replicates to estimate regularization parameters.
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Additionally, the reconstructed ANC sequence allows us to estimate lineage-specific selection.
We estimate lineage-specific mutation rates and substitutional asymmetries of transcribed regions based on repetitive elements, which lie within the intronic regions of the gene.
For nearly 20 years, nonhomogeneous, nonstationary Markov models of nucleotide substitutions have been developed and implemented to estimate lineage-specific substitution rates for an underlying phylogenetic tree (Galtier and Gouy 1998; Arndt 2007; Dutheil and Boussau 2008).
Given the problems associated with these approaches, an alternative strategy to date species trees when internal calibrations are not available in shallow species trees could be to estimate lineage-specific mutation rates for each locus under study.
To estimate lineage-specific evolutionary rates for each branch of the Glyptosternoids and their closest relatives, the CODEML program in the PAML package [ 59] with the free-ratio model (model = 1) was run on each of the 12 protein-coding mitochondrial genes.
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