It is possible that some lizards were not captured on the exact day that they emerged but, as the study site was monitored every day, our estimated emergence date should be highly correlated with the actual date of emergence.
We describe additional parameters used to estimate the emergence rate in Table 4.
Still, with our definitions we provided an upper and lower boundary to estimate complex emergence.
Alternatively, we could use field estimates of the number of host-seeking mosquitoes to estimate the emergence rate with a similar algorithm.
We therefore use the periodic sequence for population-level data of EIR to estimate the emergence rate, as described in the following section.
Earlier, we supposed using these parameters for woody plants to estimate the emergence rate from winter dormancy in spring (share of blossom buds) and vegetation period termination (share of fallen leaves) in autumn (Erofeeva 2011).
We estimated plant emergence (>50% emergence) on October 6 and 7. We estimated plant densities on October 13 by counting all the plants in the four middle rows along a 1-m long meter stick in five different regions in the 100 foot long plots.
Each bacterial strain was grown in LB media supplemented with IPTG-induced spent culture media of recombinant E. coli strains expressing or not expressing cloned autoinducer synthase genes, before estimating the emergence of resistant derivatives by exposing to phages.
Some of the assumptions the authors make in estimating these emergence dates are also problematic, says Philip Donoghue, a paleobiologist at the University of Bristol in the United Kingdom.
Four calibration points were invoked: we conservatively estimated the emergence of two main lineages at an upper limit of 3 Mya, and restricted the evolution of Gregory Rift montane isolates to the onset of the last glacial cycle, approximately 110 Kya.
