A field engineer can now estimate cell coverage without manual operations using this simple coverage estimation program.
To estimate cell response dependence on the substrate properties, the PC12 cells were cultured on Gr-AP scaffolds.
The parameters used to estimate cell damage were the total and viable cell count of the population, the proliferating ability and the cytoskeletal structure of the treated cells.
The pellets were washed twice in 0.9 % NaCl and dried at 60 °C for 48 h to estimate cell dry mass as described earlier (Sharma et al. 2012).
The DNA content was measured (based on OD 260 nm) and the DNA/cell value was subsequently used to estimate cell number.
Plates were incubated for 24 h at 37 °C and colony forming units were enumerated to estimate cell viability (Velliou et al. 2011).
Absorption properties of extracellular matrix (ECM) were investigated for carbon negative-ion-implanted polymers of polystyrene (PS), polydimethylsiloxane (PDMS) and poly-lactic acid (PLA) to estimate cell attachment properties of the modified surface without cell culture.
To estimate cell surface fluctuations, the measurement time of the I D curve was checked against a known standard [16, 18, 19]. Figure 2b shows the representative I D curves for L929 cells with different CPFA's.
We provide a practical review of the opportunities made available by design-unbiased stereology to estimate cell number, total volume, mean volume and mean height in the rat stomach using enterochromaffin-like cells as an example.
Incorporation of the thymidine analog bromodeoxyuridine (BrdU) was used to estimate cell proliferation in two functional areas of the lens epithelium: the mitotically active germinative zone (GZ) and the less proliferative center zone (CZ).
Such an accurate model, able to estimate cell thermal behavior, is beneficial to the design of Li-ion batteries, development of stress or strain sensors, optimum sensor placement, and thermal management of not only single battery cells but also battery packs.
