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I walked a full 360 degrees around the establishment to verify.
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It is important to verify establishment of this core technique.
The cells were sorted for GFP expression 7 days post-infection, and monitored for GFP expression for one month prior to analysis to verify establishment of a persistent infection.
In addition, the establishment of systematic experimental platforms to verify the theoretical methods will be another important research direction.
Eight weeks post-surgery, rabbits were subjected to a densitometry to the vertebral column and tibia bone to verify the establishment of osteoporosis model [21].
Feed consumption was recorded every 2 h from 0800 to 1400 h for 3 consecutive days to verify the establishment of an oro-sensorial preference for sweet feed (SF).
Two days after injecting the cells, mice were imaged to verify tumor establishment.
To verify emphysema establishment and severity, alveolar destruction was determined by the number of times that a predetermined group of coherent lines (1.25 mm of area and 1.50 mm of total length) crossed the parenchymal structures.
Then confirmation experiments to verify the successful establishment of these models were conducted by detecting the cellular and lobular architecture, and liver function indexes using hematoxylin-eosin staining, Masson's trichrome staining and microplate method, respectively.
In addition, each fat pad was whole-mounted and stained with aluminum carmine to verify mammary gland establishment.
The expression of β-arrestin1 and β-arrestin2 in stable K562- β-arrestin1 (β1), K562-si β1 (si β1), K562- β-arrestin2 (β2), K562-si β2 (si β2) and K562-Ctrl (Ctrl) cells were measured by real-time RT PCR, IF and WB (Supplementary Figure S1) to verify the successful establishment for further experiments.
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