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On the other hand, in the case of shrublands, the limitations for invasion could be explained by the following limiting conditions: (a) shallow ground, exposed rock, and steep slopes; (b) positioning on top of the hills, which decreases the probability of seed arrival; and (c) exceedingly dense ground layer that prevents the establishment of specimens.
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The establishment of a specimen bio-repository is also a valuable asset for the country.
Establishment of primary cultures from all specimens was achieved successfully.
The establishment of PDX models from NSCLC specimens has been recently investigated as a potential source of therapeutically relevant information and as a supply of biological material 20– 20.
The method proved to be technically simpler, faster and with a higher rate of success for the establishment of tumor primary cultures from surgical specimens than the established for previous isolation procedures [12], [14].
The use of patients' surgical specimens for the establishment of cell lines for in vitro and in vivo research had the written informed consent from the patients and was approved by the Brazilian Ministry of Health Ethics Committee, under Institutional Review Board IRB -- Researconsentcs Committee of Hospital Universitário Clementino Fraga Filho) coNo.nt CEP-HUCFF No. 002/01.
Standardization of specimen processing and the establishment of a comprehensive quality control program make the GEP70 assay highly suitable for the routine diagnostic clinical setting.
Dissections, specimen photography, the establishment of spatial coordinates for anatomical landmarks, and Procrustes transformations of landmark data largely follow Cooper and Westneat [21].
In the present work, electron microscopy and in-depth rotational analysis studies of negatively stained specimens has allowed the establishment of conditions that govern the transition between the two different rotational symmetry states (C3 and C6) of DnaB.
My spirit renewed, I dove into the folders of specimens.
The establishment of primary cultures was successfully attained from all surgical specimens.
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