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For example, Ying Cai was involved in the establishment of method to directly screen the producing strain of nisin; Haiqing Hu cloned the complete gene cluster of nisin; Jing Yuan constructed nisin mutants with broader antimicrobial spectra (Yuan et al., 2004), and so on.
Establishment of method for precise enumeration of immature chloroplasts in mitotic cells would be helpful to further investigate this issue.
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The establishment of methods for understanding the objective kinematic changes in the hip joint following ACH will provide evidence of the efficacy of surgery (Bedi et al. 2011).
The establishment of methods to control the fluctuation in gene expression is an indispensable step to the synthesis of robust and reliable genetic modules.
With the recent growing increases of wireless devices, radio spectrum scarcity occurs and mandates the establishment of methods for developing technical description to access efficiently the available radio spectrum.
The second step, development, consists in the creation of procedures and materials, the establishment of methods, and in the assessment of their acceptability by the participants (Murta et al. 2014a).
Consequently, the biosynthetic pathways to various representatives of this group of natural products were intensely studied, and the results led to the establishment of methods that allowed the generation of a plethora of artificial drugs, often called hybrid or unnatural natural products.
Therefore, in order to cure breast cancer, the mechanism of its metastasis must be fully understood to facilitate the establishment of methods to suppress the metastasis.
This should facilitate the optimization of product titer and quality in recombinant CHO cells and furthermore enable establishment of methods to screen for stably producing cell lines.
While recent advances in tumor immunology have led to the establishment of methods of generating significant clinical anti-tumor immune responses, outright tumor rejection has proved elusive in most cases.
But instead of focusing on the establishment of methods to increase the percentage of tumor-initiating cells in artificial xenograft transplantation models with immunosuppressed mice, should we not answer the question which cells initiate tumors in healthy organisms with intact immune systems as well as resist cancer therapies and causing relapses, respectively?
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com