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To further test whether establishment of expression of Hoxd1 needs both Hoxd1 and XMeis3, sub optimal amounts of morpholinos against both messengers were co-injected and injected separately.
Additional experiments are needed to distinguish between the two possibilities but whatever the outcome, this finding sheds new light on the initiation and establishment of expression of the early gastrula Hox cascade.
Is this pattern (i.e., the rapid establishment of expression divergence of paralogous genes) also applicable to A-superfamily conotoxin genes?
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The change in Igf2r and Airn expression indicate a lineage- and stage-specific establishment of imprinted expression during normal development.
The ancestral bilaterian state may have resembled the current vertebrate mode of regulation, where PcG-mediated silencing of Hox genes occurs before their expression is initiated and is responsible for the establishment of individual expression domains.
Thus, in the regulatory machinery of vertebrate Hox genes, PcG-mediated silencing occurs before Hox gene expression is initiated, and it is responsible for the establishment of individual expression domains.
To further test this synergy, and to test whether XMeis3-mediated Hoxd1 autoregulation is involved in the establishment of Hoxd1 expression, we wished to investigate the necessity of Hoxd1 for maintaining Hoxd1 expression in mesoderm.
Taken together, the observation that DNA methylation dynamics are the same in embryonic and extraembryonic tissues but that allelic expression patterns are different, require an embryonic lineage-specific mechanism responsible for establishment of imprinted expression.
In contrast, analysis of met LacZ expression confirms that met is required for its own expression in the C7-C8 brachial pool, signalling via either PI3K or Src being equally efficient to ensure establishment of met expression domain.
However, the recent establishment of recombinant expression systems for cytoplasmic dynein, together with structural and functional analyses, has advanced our understanding of the molecular mechanisms of dynein motility.
Poised enhancers marked by H3K27me3 in pluripotent stem cells have been implicated in the establishment of somatic expression programs during embryonic stem cell (ESC) differentiation.
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