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In the absence of the cohesion establishment factor Eco1, re-association of sister centromeres after spindle disruption was significantly reduced.
A similar scenario was suggested for the interaction of the budding yeast cohesion establishment factor Eco1 with PCNA.
Therefore, chl1Δ, but not the other establishment factor deletions, caused a marked synthetic growth defect in the absence of eco1.
We therefore compared Eco1 levels between wild type and cohesion establishment factor mutant strains, after arrest in early S-phase using HU.
To investigate this, we introduced the wpl1Δ deletion into strains lacking each cohesion establishment factor and again measured sister chromatid cohesion after cell synchronization.
We also show that re-association of sister centromeres after transient splitting correlates with reloading of cohesin and is promoted by the sister chromatid cohesion establishment factor Eco1.
Similar(44)
It is therefore conceivable that cohesion establishment factors act at the level of either promoting acetylation or by establishing protection from deacetylation.
We discuss the interpretation of individual-year and seasonal plant establishment factors and offer a mechanistic model for interpreting planting date and year effects on initial seedling establishment.
Others are accessory, loading, or establishment factors.
This adverse effect was maintained even after the FRD model establishment factors were terminated.
This revealed that each of the cohesion establishment factors makes a contribution to cohesin acetylation.
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