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We report the establishment and validation of three real-time fluorogenic (TaqMan®) multiplex PCRs for the detection of C. perfringens α-, β2-,β2-, ɛ-, entero- and ι-toxin genes.
Because biochemical analyses are often hampered by the amount of available diseased tissue, we report on the establishment and validation of a luminescence-based proteasomal activity assay applicable to 5-mg quantities of skeletal muscle.
More research is therefore required, examining both the process and patient outcome measures, also focussing on the establishment and validation of a morbidity and/or mortality score based on the dispatch criteria of this Flying Squad programme.
One major part of the DioRAMA project focused on the establishment and validation of cell-based bioassays for the detection of DLCs in sediments (Eichbaum et al. [16]), while in the second main experiment within the project, the time- and concentration-dependent uptake of DLCs and their associated effects were investigated in rainbow trout (Brinkmann et al. [173]).
Establishment and validation of SRM-assays.
Establishment and validation of AdnaTest BreastCancer™ have been described in detail elsewhere [ 7, 8, 11].
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In order to achieve accurate measurement of the total protein by biuret method, a detailed description of establishment, validation and uncertainty evaluation of this method is proposed in the metrological view in this paper.
A vital component in PD biomarker validation is the establishment of its pretreatment range and inherent biovariability, in order to assess the likelihood that the technology of choice and trial design have the statistical and technological resolving power to discriminate between normal background biologic variation and a drug-induced effect (Lee et al, 2005).
Mechanisms to transfer and hold data from across cohorts and other partners will include the identification of agreed data sets, specifications for the data warehouse, data validation, cleaning and harmonization procedures, and the establishment of robust data security mechanisms.
Development and validation of the Biphasic Alcohol Effects Scale.
Design and validation of the PITCh-gRNAs.
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