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After establishing the expression pattern of ABCG2 in mouse ES cells, we next investigated its possible function.
Those that were identified with this approach were further studied by establishing the expression pattern in N5 and N6, and by investigating the effect of 20E alone and that of 20E plus JH in their expression.
One impediment we encountered while attempting antibody/fluorescence-based methods of establishing the expression pattern and level of subdued was the high autofluorescence in the gut, as well as the tendency for non-specific adherence of antibodies to the gut wall.
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First, we established the expression pattern of the downstream effectors of BMP signalling in our culture system.
We used whole mount In situ hybridisation to establish the expression pattern of Rmst in E9.5 and E10.5 mouse embryos.
Once established, the expression pattern of these gradients and of the morphological substrates upon which the gradients act then diversify (Figure 1).
To establish the expression pattern of the identified mucins and mucin-domain containing proteins RNA in situ hybridization on whole-mount embryos was performed as described in Tonning et al 2006 [17].
To corroborate these findings we first established the expression pattern of p38MAPK isoforms in HL-1 cells by quantitative real time PCR.
We wanted to establish the expression pattern of eEF1A2 in ovarian cancer of defined histological subtypes at both the RNA and protein level, and to establish the mechanism for the overexpression of eEF1A2 in tumours.
The aim of this study was to establish the expression pattern of VEGF, HIF-1 α, HIF-2 α and CA9 in a series of pancreatic endocrine tumours and to correlate the level of expression with clinicopathological charateristics, angiogenesis and survival.
In order to establish the expression patterns of lamins A/C and B during hair cycling, we began by defining the natural hair follicle cycle in FVB/NCrl mice.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com