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The results suggest mechanisms establishing specificity and order in Sumo conjugation cascades.
While amenable to establishing specificity of binding, these reaction conditions were not suitable for determining reaction rate and equilibrium constants.
We found that only SV replenishment was impaired in syt 7 KO neurons, establishing specificity for the reported phenotype.
These data indicate that in both the ancestral and derived proteins, epistasis between the two variable nucleotide positions in the DNA target played a key role in establishing specificity of binding.
Instead, our data show that the temperature of the RT-reaction (50°C vs. 44°C) is more important in establishing specificity than the initial heat denaturing of the RNA.
Similar(55)
To describe an event analysis (EA) for monitoring Heidelberg Retina Tomograph (HRT) progression and to establish specificity, detection rate, and agreement with visual field progression by application to longitudinal data.
Melt curve analysis (50°C to 99°C were performed to establish specificity of amplification).
Thus, several structural differences between E1ufds, and corresponding distinctions between their cognate E2s, may help establish specificity of E1-E2 interactions.
Primers and probe are listed in Table 5. Quantitative two-step RT-PCRs were established (specificity, sensitivity, efficiency) for ORF73, Ov9, and intergenic RNA as described previously [82].
Calpain activity was measured in live dermal MVECs with an established fluorescent calpain substrate 7-amino-4-chloromethylcoumarin, t-BOC-L-leucyl-L-methionine amide (CMAC, t-BOC-Leu-Met; from Invitrogen) [66]; and calpain inhibitors, as indicated, were used to establish specificity.
To establish specificity of the primers for the HB-1 and ACC-1 alleles, mismatches to the gene sequences were introduced in the primer sequences (shaded nucleotides in Table 1).
Related(20)
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