Sentence examples for establishing beta cell from inspiring English sources

A detailed analysis of endocrine precursors in wild-type and morpholino-treated embryos using a NeuroD EGFP transgenic line revealed cooperative activity of hb9 and pdx1 in establishing beta cell fate.

Tissues were either fixed and used for immunohistological analysis or for in situ hybridization or used to establish beta cell lines.

To establish pancreatic beta cell lines, grafts were removed, dissociated and transduced with viral vectors expressing the neomycin resistance gene under the control of the insulin promoter for further selection of insulin-transcribing cells by culture in the presence of G418 (Fig. 3A).

Several Cre transgenic mice have already been established for beta cell-specific gene recombination.

It is established that beta cells produce bioactive IL-18 in the course of insulitis and actively contribute to the exacerbation of inflammation leading to their own demise [ 43].

Reduced expression of several transcription factors that play overlapping roles in establishing and maintaining beta cell functionality [ 5, 6, 8– 127 27, 44] was significant at P1 in the islets of Aldh1b1tm1lacZ null mutants whereas misregulation of other beta cell functional components was manifested mostly later.

It is now established that the beta cell mass depends on: (1) the size and the proliferative potential of the PDX1+ progenitor pool [ 31, 32]; (2) the potential of pancreatic progenitors to differentiate into beta cells; and (3) the proliferation of terminally differentiated beta cells [ 33].

"Transdifferentiation of alpha cells into insulin-producing beta cells is a very attractive therapeutic approach for restoring beta cell function in established Type 1 diabetes," said Andrew Rakeman, PhD, the director of discovery research at JDRF, an organization that funds research into Type 1 diabetes.

Transduced tissues were next transplanted under the kidney capsule of immuno-incompetent mice allowing insulinoma development from which beta cell lines were established.

Ryu, S., Kodama, S., Ryu, K., Schoenfeld, D. A. & Faustman, D. L. Reversal of established autoimmune diabetes by restoration of endogenous beta cell function.

A central role in mouse islet development and/or beta cell function has been established for the pancreatic-duodenal homeobox factor-1 (PDX1), MAFA and MAFB transcription factors [ 21– 27].