In 1950 he began an interdisciplinary study of child development with Milton Senn at Yale University, establishing the combination of direct observation with psychoanalytic methods as a research tool in child psychology.
In this model, we established the combination of standardised electrocautery and sutures for adhesion induction.
International initiatives have established the combination of immunohistochemistry (IHC) and in situ hybridization as the current gold standard [ 9, 10].
ECOG 4599 was the first of these trials and established the combination of bevacizumab and cytotoxic chemotherapy as a new standard of care for eligible patients [ 13].
For the analysis of compartment-specific analysis of gene expression in human RA synovium, we established the combination of laser-mediated microdissection and differential display to analyze distinct gene expression profiles of histologically defined areas in RA synovium.
Our study was conceived and planned before the initial presentation of the ABC-02 trial results in June of 2009, which established the combination of gemcitabine and cisplatin as a new standard of care for patients with advanced biliary cancers.
Different chemotherapeutic agents have been employed [ 3]; few randomized trials have established the combination of gemcitabine (GEM) and platinum compounds to be the standard of therapy for unresectable BTC patients [ 4– 6].
The MOSAIC trial, a milestone publication [ 6], established the combination of 5-fluorouracil (5-FU), leucovorin and oxaliplatin (FOLFOX4) as the standard adjuvant chemotherapy regimen, replacing 5-FU and leucovorin in UICC-stage III colon cancer-patients after R0 resection [ 6, 7].
Although still on an experimental level, with many parameters still to be considered, this (and other recent reports) example shows that once a reliable and stable cell culture system has been established, the combination of whole genome analysis and in vitro cell culture can contribute to early detection of specific target organ toxicities.
In addition, a prediction model was established using the combination of p300, MYST4 and HDAC1, with the accuracy of 0.9338.
