To formally establish whether the interaction between AA and CyP-D determines PTP inhibition, we purified mitochondria from either wild-type or Ppif−/− (CyP-D null) mouse fibroblasts Figure 3A and [27].
To establish whether the interaction of each lobe observed was genuine or a consequence of the mutation in the other lobe, the Ca2+ dissociation kinetics was examined using CaMWT.
In order to establish whether the interactions between Dsg3 and E-cadherin that we had detected by confocal microscopy and co-immunoprecipitations were indeed real we used the acceptor photobleaching FRET technique [38].
It remains to be established whether the 5-HT/AS interaction is either pathological or physiologically relevant in vivo.
Furthermore, to establish whether the observed interaction between ORF57 and PYM was direct, GST-pull-down experiments were performed using purified baculovirus-expressed recombinant ORF57-6xHis protein and a negative control baculovirus-expressed protein, HIV-Nef-6xHis (Harris and Coates, 1993).
In this regard, it remains to be established whether the suppression of microtubule dynamics account for this synergistic interaction.
To establish whether this interaction occurred during monocyte activation, we immunoprecipitated FOXO3 from stimulated monocytes and found that the DNA to which it was bound included the predicted binding site from the TGFβ1 promoter, confirming that a direct protein-DNA interaction occurs.
Consequently, wider sampling is still needed to help establish whether the Hox-TALE interactions characterised by Hudry et al. really did originate with the cnidarian-bilaterian ancestor or if, in fact, they were established even earlier.
To establish whether the NTD directly mediates interaction with TARPs, we first used immunoprecipitation (IP) to test if the NTD contributes to stabilizing the AMPAR-TARP complex.
It remains to be established whether characterizing interactions between widely distributed neuronal populations may give a more accurate representation of dynamic clinical phenotype, particularly given the consideration that recording from more than one site raises practical implications such as increased risk of hemorrhage and infection.
