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Spring wheat (Triticum aestivum) was established in 2013, either by conventional ploughing (CP) or direct drilling (DD); and winter barley (Hordeum vulgare) established using the same methodology the following autumn 2013 and harvested in 2014.
Both cultures were established using the same method and grown for approximately the same number of population doublings.
Stable clones were established using the same procedure as for T24 cells.
Gene expression profiles were established using the same parameters as in WT1 knockdown experiments.
The numbers of chicken miRNA*s, and miRNAs and miRNA*s of other metazoans, were established using the same criterion.
In addition, pGL3-GG/TT containing SNP rs4648065 (C > T) was established using the same method to investigate the co-effect on rs4648068.
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Once violet cone spacing is complete, these cells would turn off expression of the molecules mediating lateral inhibition, and the next most abundant cell type (blue cones) would establish spacing using the same mechanism.
Incidentally, our average FE NO values were within the limits of reported reference values established using NO analyzers of the same manufacturer [ 21, 31].
The total technical variation was between 2 and 8 percent, depending on compound, as was established using 12 extractions of the same frozen powder from a randomly chosen genotype.
The groups were established using callow sister workers of approximately the same size from each colony and placing them in small nest boxes.
Therefore, to further understand the complexity of P. aeruginosa swarming migration, microarrays gene expression profiles of bacterial populations localized at the tip of swarming tendrils and swarm centers were established using for control bacteria grown in the same culture conditions but on plates dried longer to prevent swarming motility [ 20].
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