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A diagnosis of disease was established using published criteria.
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Human iPSC lines were established using the published protocols[22], [23], [25].
The diagnosis of POEMS was established using the published criteria.
Primers for qPCR were designed for established genes using published rat sequences and PrimerExpress software for Real-Time PCR (version 3.0; Applied Biosystems) (Table 2).
RPE differentiation was then established using a variation of a previously published protocol (Meyer et al., 2009).
Primary culture of human nasal epithelial cells was established using an adaptation of the methods published by Nlend et al.[ 8].
Using published evidence on established risk factors, we developed an interactive, personalized knee OA risk calculator (OA Risk C) and conducted a pilot study to evaluate its acceptability and feasibility.
The chemical structures were established using NMR spectroscopic, mass-spectral analyses, and published data.
The acoustic pressure and ultrasonic intensities (IPA and ITA) were calculated using published equations and technical standards established by the American Institute of Ultrasound in Medicine and the National Electrical Manufacturers Association [62].
A unit cost representative of diverse payers was established using fee schedules [ 25, 26] and published payer ratios [ 27], and applied to each CPT-4 code in the profiles.
In these case studies the activity composition relationships were established using Artificial Neural Networks (ANNs) trained with catalytic data published earlier.
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