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To develop an assay more suited to high-throughput genotyping and to give quantitative information on copy number, a high-resolution melt curve assay was established using a pair of primers designed to amplify a common amplicon of 70 bp from both α- and β-tryptase sequences but with six-bp mismatches between them.
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To develop a rapid and reliable detection method for Citrus yellow vein clearing virus (CYVCV), a quantitative real-time reverse transcription-polymerase chain reaction (qRT-PCR) system based on SYBR Green I was established by using a pair of specific primers designed from its conserved coat protein gene.
To establish the portals, a sharp incision (0.5 cm) was made at the mark, followed by blunt expansion toward the coracoid process using a pair of straight tongs.
I tried using a pair of mating radiateds.
Try using a pair of Channellock pliers.
Finally, an SVM model is established using these feature vectors of the protein pair as input.
I could use a pair of shoes".
Use a pair of scissors.
Use a pair of barber scissors.
This example uses a pair of socks.
The PCR amplification efficiency of each primer pair was established using calibration curves (Additional file 2).
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