Sentence examples for established transposition from inspiring English sources
Setting aside the exact reasons why new tubulin loci were established, transposition events in the ancestor of Leishmania may have been essential to overcome the historical constraint inherited from non-parasitic kinetoplastids, and facilitate the evolution of novel expression patterns.
Parallel plating of the transformation on LB agar with Ampicillin alone was used to establish the transposition efficiency.
Having successfully established the targeting and transposition steps of the labelling, we employed CLoNe in mouse and chick forebrain development to reveal clonal relationships.
When the calculated diffusion rates are applied in the computer model, a pseudo-steady-state rate of transposition is established when there are about 200 copies of the transposon.
The full size of the male-specific region and an accurate estimate of the age of the transposition event that established it are beyond the scope of this analysis.
The absence of regional and sequence preferences of IS 6100-transposition demonstrate that the established system is suitable for efficient genome-scale random mutagenesis in the sequenced type strain C. glutamicum ATCC 13032.
Also considering the diversity of ORFs interrupted by IS 6110 copies, gene knock out studies and assessment of downstream gene expression are necessary if a functional role for specific transposition events is to be established.
This study also established a linkage between the intI1 and Tn21 transposition genes, in 18/27 E. coli isolates from bovine sources and 2/4 isolates from human sources, which implicates Tn21 in the distribution of this novel integron.
The mobility and transposition functions of piggyBac have been established and exploited to develop an important binary system for insect germline transformation [ 3].
Like Mos1, the enzyme forms a dimer in solution, and this was recently established as a prerequisite for autoregulation in mariner transposition.
Accordingly, the target site selection of Mu transposition is optimally suited for the purpose established in this study.
