A cell line is a permanently established, transformed clonal lineage, where the daughter cells will proliferate indefinitely when given proper medium and growth substratum conditions.
The investigation included cell lines with several years of continuous in vitro culturing along with freshly established transformed B-cell cultures of only 3 weeks of age.
Far from a Monopoly game in which people deploy stylized cash in pursuit of their own individual advantage, we find participants investing effort in establishing, transforming, and sometimes terminating interpersonal relations" (Zelizer, 2014, p6).
Further investigation should be carried out to establish transformed insect cell lines with stable expression of corresponding interfering RNAs.
However, we were not able to detect any changes in genes associated with DNA repair as reported by Rodrigues et al. The difference is likely due to the strategies used to establish transformed cell lines.
A culturalist strategy was established for transforming the ancient values of sylvanic trees into some modernized environmental and cultural values.
RGC5, an immortalized RGC cell line established by transforming postnatal day 1 rat retinal cells with E1A adenovirus [39], has been used widely and extensively as a model of RGC for various investigations [24], [40].
To obtain a steady source of DNA for genetic studies, cell cultures of LCLs were established by transforming each donor's B cells with the B95-8 EBV strain.
The rat RGC line (RGC-5), established by transforming rat postnatal day 1 retinal cells with the 2Ψ E1A virus, was purchased from the American Type Culture Collection (Manassas, VA, USA).
To measure the inter-patient similarity consistently, the feature vector model has been established by transforming the clinical information of EHRs, including laboratory test findings, medical images and diagnostic reports, to vector elements systematically [ 3– 6].
