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By crossing Actb-MBS and MCP mice, a double homozygous mouse line, MCP × MBS, was established to visualize endogenous β-actin mRNA labeled with multiple green fluorescent proteins.
PDMPO has successfully been established to visualize silica in diatoms and other organisms.
Moreover, maps of structured spatial effects at the district level were established to visualize the geographic distribution of RR.
Generally, real-time RT-PCR was used for the detection of ANXA1 mRNA expression, while Western blotting and immunohistochemistry were established to visualize the tissue-specific expression pattern and quantification of ANXA1 protein in these specimens.
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It is already an established method to visualize R. eutropha H16 cells and granules under different growth and PHB production conditions ([Tian et al. 2005a, 2005b]).
This is attributable to the difficulty in generating 3-D matrices with controlled morphology, rheology, and matrix composition, and a lack of established methods to visualize and evaluate cell functions over prolonged periods.
To this end we have established an approach to visualize and manipulate stem cells and their niche in the skin of an intact, uninjured mouse.
We previously established a method to visualize neural activity in the honeybee brain, using a newly discovered immediate early gene, which we termed kakusei [8].
To gain insight into the cell cycle dynamics of Lgr5+ CBCs, we have established a system to visualize actively proliferating cells using the proliferation-specific expression of the mKi67 gene and characterized the early fate choices of intestinal stem cells.
Users had to distinguish which information was worth mapping and establish the parameters to visualize their data.
This type of complex scenarios, where bacterial resistant populations and their genetic platforms containing AR genes move because they are selected, and evolve because of their promiscuous migration, establish the precondition to visualize AR not only as a matter of biological function, but also as information flow processing.
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