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NW co-designed and established the screening platform, performed the screening of the strains, analyzed screening data, extracted DNA from target strains, and reviewed drafts of the manuscript.
UMT conceived and designed the experiments, performed the in vivo selection on the mutIV-mix, the bioreactor validation of the selected strains, the PCRs for Sanger Sequencing, the 454 Sequencing runs (together with Markus Grohme), analyzed NGS and Sanger results, co-designed and established the screening platform, and wrote the manuscript.
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KLHF, HK, and AB established the screen; KLHF performed the GSK3β experiments; UL performed the ING5 experiment; AHF, NH, and PV purified ARTD10; EK generated the mAbs; BL supervised the work; KLHF and BL wrote the manuscript.
However, Deighton et al. [ 9] also recognised that assessing the properties of the measure in a clinical population would be essential towards establishing the screening capacity and clinical usefulness of the measure.
The plasmid pIκB EGFP, which contained a chimeric gene that encodes a fusion protein, green fluorescent protein (EGFP) fused with IκB-α (IκB EGFP), was used to establish the screening system.
After establishing the screening protocol using human sera, eight unique oligopeptide phage clones were selected, showing significant reactivity to whole sera as well as enriched anti-tick specific antibodies from three tick-bite sensitized donors.
Establish the Screen GameThe best way to slow an opponent's pass rush and open up deep opportunities for wide receivers is to establish the screen passing game.
This component used the direction of eye or head to establish the screen-space coordinates of where the user was looking.
To establish the screen and define the magnitude of miRNA-mediated effects, we first sought a control miRNA that directly targets ErbB receptors.
To differentiate between cardiac and renal phenotype, we established the following screening assays for zebrafish.
However, previous work has already established the feasibility of screening in cancer settings, and the superiority of screening with triage to screening without triage (Carlson et al, 2010), so it seemed somewhat unethical not to offer some form of triage.
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