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In Italy, a Kafkaesque approval process in which the Agriculture Ministry has never established the requirements for success, makes genetically modified crops a nonstarter.
GCOS has defined a set of Essential Climate Variables (ECV), established the requirements for their systematic observation, and the development of data archives, needed to support the study the climate system.
However, if a mechanism of action can be established, the requirements for epidemiologic evidence outlined above can be somewhat relaxed.
DOI: http://dx.doi.org/10.7554/eLife.06659.007 Next, we established the requirements for the formation of interconnections between Smc ScpAB rings and chromosomes.
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When we consider social entrepreneurs for the Schwab Foundation network, we therefore established the requirement that the venture has to be minimum 3 years old.
Some two decades later Title III of the Electronic Communications Privacy Act (1986) established the requirement for a court order to obtain this type of data, either under 18 USC 3123, or under the Foreign Intelligence Surveillance Act of 1978 (50 USC 1801).
Having established the requirement for NO production, we next analyzed the role of PtdIns 3-kinase in NO production.
Western blot analysis of cytoplasmic and nuclear fractions from cells transiently transfected with T423E-Pak1 constructs further established the requirement of LC8 for Pak1 nuclear import (Fig. 2E).
Our data clearly established the requirement of GCLC for the development of TSC-related phenotype.
This is consistent with the recent findings of [57] in proliferating EC who established the requirement of STIM1/Orai1 for CCE.
Control experiments established the requirement for catalytic Ru and Ag (entries 7 and 8) and stoichiometric Cu OAc 2⋅H2O (entry 9).
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