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To aid our analysis, we first established the expression patterns of two known cardiac genes; in situ hybridization was performed using probes for Nkx2-5 and Isl1 at the corresponding time points (Figure 5 A F).
Having established the expression patterns of the PYK1, PFK2, PCK1 and ICL1 fusions in vitro, we examined their behaviour in two medically significant ex vivo models.
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First, we established the expression pattern of the downstream effectors of BMP signalling in our culture system.
Once established, the expression pattern of these gradients and of the morphological substrates upon which the gradients act then diversify (Figure 1).
To corroborate these findings we first established the expression pattern of p38MAPK isoforms in HL-1 cells by quantitative real time PCR.
In order to establish the expression patterns of lamins A/C and B during hair cycling, we began by defining the natural hair follicle cycle in FVB/NCrl mice.
Further research will be needed to establish the expression patterns and functional significance of CTIF in the inner ear.
In the present study, we sought to establish the expression patterns and possible functions of the α6A and α6B integrin subunits in the normal human and adenocarcinoma cells of the colon.
We used whole mount In situ hybridisation to establish the expression pattern of Rmst in E9.5 and E10.5 mouse embryos.
After establishing the expression pattern of ABCG2 in mouse ES cells, we next investigated its possible function.
To establish the expression pattern of the identified mucins and mucin-domain containing proteins RNA in situ hybridization on whole-mount embryos was performed as described in Tonning et al 2006 [17].
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