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First, we established the expression pattern of the downstream effectors of BMP signalling in our culture system.
Once established, the expression pattern of these gradients and of the morphological substrates upon which the gradients act then diversify (Figure 1).
To corroborate these findings we first established the expression pattern of p38MAPK isoforms in HL-1 cells by quantitative real time PCR.
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To aid our analysis, we first established the expression patterns of two known cardiac genes; in situ hybridization was performed using probes for Nkx2-5 and Isl1 at the corresponding time points (Figure 5 A F).
Having established the expression patterns of the PYK1, PFK2, PCK1 and ICL1 fusions in vitro, we examined their behaviour in two medically significant ex vivo models.
We used whole mount In situ hybridisation to establish the expression pattern of Rmst in E9.5 and E10.5 mouse embryos.
After establishing the expression pattern of ABCG2 in mouse ES cells, we next investigated its possible function.
To establish the expression pattern of the identified mucins and mucin-domain containing proteins RNA in situ hybridization on whole-mount embryos was performed as described in Tonning et al 2006 [17].
We wanted to establish the expression pattern of eEF1A2 in ovarian cancer of defined histological subtypes at both the RNA and protein level, and to establish the mechanism for the overexpression of eEF1A2 in tumours.
Those that were identified with this approach were further studied by establishing the expression pattern in N5 and N6, and by investigating the effect of 20E alone and that of 20E plus JH in their expression.
The aim of this study was to establish the expression pattern of VEGF, HIF-1 α, HIF-2 α and CA9 in a series of pancreatic endocrine tumours and to correlate the level of expression with clinicopathological charateristics, angiogenesis and survival.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com