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For this analysis, we first validated the Nrp1 knockdown in mutants and established the expression level of Pecam relative to the housekeeping gene Actb to provide a measure of EC quantity.
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Quantitative PCR (Q-PCR) established the expression levels of key DNA DSB repair genes.
Supplementation of the culture medium with exogenous iron re-established the expression level of these genes.
To establish the expression levels of E2F3 protein, we performed a western blot analysis (Figure 11) and we observed that E2F3 was markedly overexpressed in tumor samples.
Cell lysates were then analysed directly, to establish the expression levels of the input proteins, or immunoprecipitated with monoclonal antibodies against the HA or Flag epitopes.
Further quantitative proteomic studies are needed not only to establish the expression levels of these proteins in each PD variant, but also to establish the protein levels associated with health.
To further study the results found in the iTRAQ analysis, we performed immunoblot analysis to establish the expression levels of a set of proteins in both ovarian cyst fluid and plasma (Table 3).
Functional validation experiments will be required to establish that the expression level of each gene does drive or modulate cancer progression.
An inverse relationship has thus been established in the expression levels between TMEM106B (upregulation) and PGRN (downregulation) in FTLD-TDP.
In this direction, an inverse correlation has been established between the expression levels of S100A4 and E-Cadherin [ 42] and has been associated with poor differentiation of cancer cells [ 31].
We next sought to establish if the expression levels of key proteins in the mitochondrial apoptotic pathway could be utilized to predict the observed cellular response to treatment.
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