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"established the backbone" is a correct and usable phrase in written English.
It can be used to describe the action of creating a strong or sturdy foundation or structure for something. Example: The new CEO implemented a series of successful strategies that established the backbone of the company, leading to its exponential growth over the past year.
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The landmark act led to the founding of institutions including Cornell, Berkeley and the University of Wisconsin, and established the backbone of the finest public university system in the world.
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After designing and composing the piano lead sound, I wanted to quickly establish the groovy backbone that I'd been hearing in my head.
Several controls in these studies, such as reverse and scrambled peptides as well as MAP backbone immunization, have established the specificity of the immune response to Ro273 289 [18].
And Hinman notes that the Chinese government also received cooperation from other American companies, such as Cisco Systems, in establishing the infrastructure or backbone components of its Internet firewall.
The dollar is the world's reserve currency and, for over 200 years, we have established ourselves as the backbone of the global financial system.
For most of our networks, we use the noise-corrected backbone, which establishes the expectation for an edge weight and keeps it in the network only if the actual edge weight is higher than this expectation [20].
The single-crystal X-ray diffraction analysis of a nonchiral β-Ala-containing model peptide, Boc-β-Ala-Aib-OCH3 1 (β-Ala, 3-aminopropionic acid; Aib, α-aminoisobutyric acid), establishes the coexistence of distinctly different backbone conformations in two crystallographically independent molecules, A and B, in the asymmetric unit.
It also firmly establishes the existence of a common backbone between pAW63, pXO2 from Bacillus anthracis and pBT9727 from the pathogenic Bacillus thuringiensis serovar konkukian strain 97-27.
This study defined the constituent subfamilies of the PACMAD clade and established a backbone phylogenetic hypothesis against which deeper phylogenetic relationships could be explored.
SNiPA includes a wide range of genome-level datasets contained in the Ensembl database (Flicek et al., 2014) as an established backbone of annotations for the human genome.
In other words, in order to implement a determined backbone sequence it is sufficient to properly establish the conditions.
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Since I tried Ludwig back in 2017, I have been constantly using it in both editing and translation. Ever since, I suggest it to my translators at ProSciEditing.

Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com