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The validation also established the assay to be stability indicating, by showing that changes to the integrity of the PRP-OMPC conjugate could be detected.
Having established the assay conditions, we observed a good correlation between IC50s obtained for PfCDPK1 and PbCDPK1 (r = 0.96; P = 0.00001) or PfCDPK1 and PvCDPK1 (r = 0.86; P = 0.001).
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The TT was also used to establish the assay reactivity threshold (RT).
Using data gathered from our study we developed a selective substrate for cathepsin S and establish the assay conditions to differentiate the enzyme from cathepsins L, B, V and K.
To establish the assay, anti-HSA antibody was first coated on the surface of NaYF4:50%Yb1%Er@carboxyl UCNPs to detect HSA target protein (Fig. 7a f).
The technique originated from the same Oxford University laboratory and a trained laboratory technician from the Gambia helped to establish the assay in Senegal.
Together, these results establish the assay as a valuable tool for measuring neutrophil motility, and provide a reference set of values characteristic of neutrophils from healthy subjects.
As the goal of the study was to establish the assay, the patient group sizes were relatively small.
Moxifloxacin administration resulted in a clinically significant increase in QTcF, thereby establishing the assay sensitivity of the trial.
At the time of establishing the assay, 25 ml acetate buffer, 2.5 ml TPTZ, and 2.5 ml FeCl3·6H2O was mixed to prepare the FRAP solution.
Of note, when enough tissue was available, both biomarkers were used to determine eligibility, with LRRC19 > IGFBP2 being the priority assay due to the fact that the preclinical work establishing the assay was published.
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