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These dynamics lead to a temporally shifting NF-κB/Dorsal gradient which refines and disappears before the major DV patterning genes have established stable expression domains.
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AP-SSTR3-GFP or AP-Smoothened-YFP was inserted into pEF5B/FRT-DEST to establish stable expression in FlpIn cells.
We first analyzed the impact of the TERT promoter mutations on TERT mRNA levels by qRT-PCR until cultures established stable TERT expression levels and all TERTΔ/Δ had died.
To investigate the effects of TGF-β family cytokine receptor mutations on endogenous telomerase activity and telomere length in breast cancer cells, we established stable gene expressions of the receptor mutants along with negative and positive controls by repeated antibiotic selection and FACS sorting for GFP-positive cells.
We also established stable clones expressing full-length HoxA9.
We established stable cell lines that express shRNA expression vector against ER-α36 (HecellsNandcexaminedd examined ER-α36 expression (Fig. 2C and 2D).
We established stable transfectants that expressed Snail or SIP1 using each expression plasmid in HepG2 and Huh-7 cell lines.
Therefore, in order to evaluate the possible mechanism of the involvement of MICA expression, we established stable human MICA-transfected corneal epithelium with a lentivirus vector expressing GFP.
The SKBR3 cells without detectable endogenous expression of Siah1 and Sial1L were chosen to establish stable gene expression.
To ensure that these observations were not influenced by the transient expression of LMP1, we established stable transfectants of the HaCaT cell line expressing B95.8-LMP1 B95.8-LMP1
To test if PyLT induces cdk2 activity, and to address the mechanism of its induction, we established stable inducible cell lines in which expression of PyLT was regulated by the addition of a small molecule dimerizer, AP1510 (AP).
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