Studies to establish specificity for the uricase protein and various PEG preparations are described in the text and figure legends.
We found that only SV replenishment was impaired in syt 7 KO neurons, establishing specificity for the reported phenotype.
To establish specificity of the primers for the HB-1 and ACC-1 alleles, mismatches to the gene sequences were introduced in the primer sequences (shaded nucleotides in Table 1).
In order to establish specificity of the observed effects for PDGFRβ-mediated signaling we additionally used an inhibitor of the PDGF-receptor kinase (AG1296, Merck) for the proliferation and migration assay at a concentration of 1 µM following manufacturer's instruction.
While amenable to establishing specificity of binding, these reaction conditions were not suitable for determining reaction rate and equilibrium constants.
Primers and probe are listed in Table 5. Quantitative two-step RT-PCRs were established (specificity, sensitivity, efficiency) for ORF73, Ov9, and intergenic RNA as described previously [82].
Therefore, BMDCs were stimulated with LPS in the absence or in the presence of the BAY11-7085 inhibitor with well-established specificity for IKK [41], [42], [43] and JunB expression was analysed by immunoblotting.
To discriminate between PP1 activity and PP2A activity, we used small-molecule inhibitors with a well-established specificity for the two phosphatases, which are standards in the literature and were confirmed in control experiments.
Recently, functional neuroimaging studies have established hemispheric specificity for a range of language, motor, and spatial tasks [ 12].
Unlike Nile red, BODIPY 505/515 is insensitive to the environment polarity [ 49] and numerous studies have established its specificity for lipid droplets.
The tripartite motif (TRIM) gene family is a highly conserved group of E3 ubiquitin ligase proteins that can establish substrate specificity for the ubiquitin-proteasome complex and also have proteasome-independent functions.
