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By means of newly established reporter mice, maximum of IFN-β expression was observed at 24 hours post infection in spleen and, surprisingly, 48 hours post infection in colonized cervical and inguinal lymph nodes.
To set up an assay for the determination of the influence of the seven selected TPR proteins CHIP, CYP40, FKBP51, FKBP52, PP5, TPR2 and XAP2 on the six steroid receptors GR, MR, PR, AR, ERα and ERβ, we established reporter gene assays for each of the receptors.
To this end, we monitored localization of 40S subunits in Tsr2-depleted cells using the established reporter uS5-GFP (yeast Rps2-GFP; Milkereit et al., 2001).
DOI: http://dx.doi.org/10.7554/eLife.10441.004 We used an established reporter gene assay (Liberles and Buck, 2006; Ferrero et al., 2012) to query the response properties of TAAR13c variants with charge-neutralizing mutations at Asp3.32 (Tand13cD112Asp5.42Asp5.42 (TAsp5.42202Asp5.42
Moreover, when the activities were compared quantitatively, the G-Luc clones displayed, on average, more than 7-fold higher bioluminescence signal intensity than the R-Luc clones (Fig. 2a), indicating that the new G-Luc is more sensitive and more efficient than previously established reporter genes for Chlamydomonas.
Although the Chlamydomonas-specific G-Luc described here provides a workable reporter of gene expression that is significantly more sensitive than previously established reporter genes, our data indicate that the superior performance of the Gaussia luciferase gene is not due to its better expression in C. reinhardtii, but rather due to its very high enzyme stability.
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By Andrew Marantz The White House is calling on young correspondents from far-right Web sites, to the exclusion of established reporters.
Not, 'What's your message today?' Not, 'Here's a paintbrush — would you paint us a pretty picture?' " If established reporters got fewer questions relative to the floaters, I asked, would this be good or bad for democracy?
"Buy My Product!" will always be a less convincing approach than getting established reporters or customers to tout a firm's services in a magazine.
This volatility in the journalism job market has driven many established reporters into other fields.
From a biological perspective, primary cells and mixed cell cultures would be preferred, but they present challenges of availability, generally require special handling, are not easily adaptable to 1,536-well 1,536-wellitions, assayre not used to establish reporter gene assays, whiconditionsute the mandrity of current qHTS areays.
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