Sentence examples similar to established protocols are used from inspiring English sources
Following deprivation from FCS and stimulation as indicated established protocols were used for total RNA preparation, cDNA synthesis and real-time RT-PCR [ 22].
Such established protocols are now widely used in pharmacological, environmental monitoring, food safety, and wastewater toxicity investigations.
Radiation dose concerns and the absence of established protocols are factors that have potentially limited the use of CT for work-up in these young children.
For all western blot experiments, established protocols were performed [ 20], using antibodies that recognize phosphorylated or unphosphorylated EGFR, FAK, c-Src, Cortactin, Paxllin, β-actin.
Previously established protocols were employed [8].
Established flow cytometry protocols were used to determine the specificity of endocytic pathway inhibition, demonstrate peptide internalization, and determine primary mechanism of endocytosis.
When mock-treated samples were below the level of detection, the protein concentration designated as the limit of detection (as established by the manufacturer's kit protocols) was used for normalization.
Standard protocols were used to establish stable CAG::mRFP1 transgenic lines of ES cells constitutively expressing mRFP1.
Standard protocols were used to establish stable lines of ES cells constitutively expressing an H2B fusion [ 20- 22].
At the first visit, a semi-structured interview (see Additional file 1) following an established protocol was taken by a pediatrician and used similarly for all subjects, to obtain a detailed personal medical history.
