Using fluorescence in situ hybridisation (FISH) approach, we established its localisation on the chromosome 11q23.2.
DOI: http://dx.doi.org/10.7554/eLife.03743.002 In most invertebrates and anamniote vertebrates (fishes and amphibians), embryonic polarity is first established by localisation of maternal determinants in the cytoplasm and/or cortex of the fertilised egg.
The primary aim of the present study was to establish the localisation of ascorbate (SVCT1/2) and dehydroascorbate (GLUT1-4) transproteinsroteins in the human bronchial epithelium and to determine whether vitamin C uptake by the respiratory epithelium played a role in the regulation of the extracellular ascorbate pool within the lung.
SSRS is widely available and well established for the localisation of primary NETs.
The role of iodine-123 labelled meta-iodo-benzyl-guanidine (MIBG) scanning is well established in the localisation, staging and follow-up of neuro-endocrine tumours especially those arising from the sympathetic nervous system, such as phaeochromocytomas and paragangliomas.
We have cloned the full-length cDNA sequence of SKCG-1, established its chromosomal localisation, determined its structural analysis and coding potential, analysed its expression in various normal human tissues, including Wilms and breast tumours and their corresponding uninvolved tissues, and tested its function in cell growth by silencing it.
However, to better support this novel relationship between the KDELR and PHB, we carried out a database analysis, with a search for any PHB interactors that have a well-established localisation to or function in the ER or the Golgi complex.
For example, it is unknown whether TACC-TOG binds to the Ndc80 internal loop in vertebrates, though it is firmly established that TACC-TOG localisation to the Ndc80 internal loop in fission yeast plays important roles in mitosis [ 42, 43] and meiosis [ 91].
A variety of fluorescence patterns were obtained and co-localisation with established cellular markers was used to tentatively identify the labelled compartments.
Further, we have performed tests to study the necessity for Smad proteins during TSC-22-induced apoptosis, and to establish the intracellular localisation of TSC-22.
Our attempts to generate transgenic lines expressing the PUF domain of other worm PUF proteins, which would have established whether they too enable nuclear localisation, did not succeed.
