In contrast, all of these well established lifespan-extending interventions reduced rDNA recombination.
Two ways to measure aging have been established in yeast: replicative lifespan (RLS) and chronological lifespan (CLS) (Mortimer and Johnston 1959; Longo et al. 1996, 2012).
NHU cell lines of finite lifespan were established and maintained in keratinocyte serum-free medium containing bovine pituitary extract, recombinant EGF and supplemented with cholera toxin (KSFMc), using methods that have been described in detail elsewhere [12], [14].
We crossed a short- to a long-lived strain, recorded lifespan, and established polymorphic markers.
It is well established that CR increases lifespan and healthspan in a range of organisms (Anderson & Weindruch, 2010), although the mechanistic driver of these effects is currently unknown.
Furthermore, the same group has established that a significant lifespan extension can be achieved by ectopic overexpression of histones in wild-type yeast.
While it is known that high life expectancy is associated with low lifespan variation, we wanted to establish whether life expectancy leaders had the most egalitarian lifespan distributions.
These results suggest that the physiological characteristics of mitochondrial function associated with lifespan determination are established during early development and persist throughout the life cycle of the whole organism.
The outcome component of this study will determine whether LIFEspan establishes continuity of care within the first year post-transition, which is an important first step in maintaining long-term health.
It therefore remains to be established how S6K orthologs affect lifespan.
Caloric restriction (CR) is the intervention most well established as able to increase lifespan in experimental models [ 2], and investigators are now seeking other interventions that may mimic the life-prolonging effects of CR without requiring a reduction in caloric intake [ 3].
