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This finding suggests that Notch-Delta signaling may represent one of the mechanisms whereby this patterning is established in zebrafish.
Subfunction partitioning can involve protein structural changes and has important functional and evolutionary consequences, but few such examples have been established in zebrafish.
MOs block translation of transcripts by steric hindrance in an efficient and gene-specific manner, which has been well established in zebrafish and other model organisms [17] [20].
More recently, the importance of FN in somitogenesis has also been established in zebrafish [29], [30], Xenopus laevis [31], [32] and chick [33].
As no stem cell marker has been established in zebrafish or human, verification of the model results still awaits future work in this field.
Previous work has established in zebrafish that expression of extracellular ependymin can be inactivated by direct intracerebroventricular injection of the antiserum, with maximal down-regulation at 6-8 hours, after which recovery occurs [19], [20].
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A functional circulatory system including a primitive heart is already established in the zebrafish embryo by 24 h post-fertilization (hpf).
The direct application of the Drosophila Gal4-UAS approach, by the generation of transgenic lines, has been established successfully in zebrafish [ 6, 7].
The new approaches include DNA microarray fingerprints in algae [21, 22], a proteomic approach in zebrafish embryos previously established in adult fish tissue [23] to direct EDA, and a metabolomics EDA approach using invertebrate and fish cell lines in vitro.
To further decipher Myc functions and cell fate decisions, genetic models utilizing inducible protein versions have been established in higher vertebrates and recently in zebrafish.
Much of the complexity of the human immune system is well established in ray-finned fish, making zebrafish a tractable model to study innate immunity and inflammation in vivo (Renshaw and Trede, 2012).
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