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Homogeneous fibroblast culture was established in serum- and calcium- containing media (Fig. 6C).
A reference interval for VEGF was established in serum and plasma from 306 healthy persons.
Therefore, NB TICs may more closely reflect the cells from bone marrow metastases as compared to NB cell lines established in serum and selected for adherent culture.
A patient-population-specific floating cut-off point was established in serum samples from over 300 IVF patients collected at various time points of the menstrual cycle.
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Primary cell cultures from two donors were established in serum-supplemented media in the presence of murine feeder cells.
Spheroid cultures were established in serum-free medium containing 20 ng/ml EGF and 10 ng/ml bFGF (PeproTech, Rocky Hill, NJ, USA) and subsequently kept on ultra-low attachment dishes (Nunc, Sigma-Aldrich, St . Louis MO, USA) in a 5% CO2 humidified incubator at 37 °C as described.
All mesothelioma cell lines derived so far were established in-high serum growth media.
The JANUS serum bank, established in 1973, includes serum samples from more than 330,000 healthy donors, of whom 91% were recruited from population-based health examinations of participants in the 35 49 year age-group.
The downregulation of the hormone adiponectin in the diabetic state has been well established in adipocytes and serum [38], but it was not until 2005 that Pineiro et al. showed evidence that cardiomyocytes also synthesize adiponectin [39].
Mouse embryonic stem cells (ESCs) were first established in fetal calf serum (FCS -containing medium with mouse embryonic FCS -containingF) feeder cells (Evans and Kaufmedium981; Martin, 1981).
The JANUS serum bank was established in 1973, and has serum samples from more than 330 000 healthy donors.
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