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We postulated a regulatory mechanism established in a cell that protects it from deleterious effects of mutations by keeping the macromolecular composition of a cell quantitatively in balance.
The ABCB1/P-gp-overexpressing drug-resistant cell line KB-C2 was established in a cell culture medium by a step-wise selection of the parental human epidermoid carcinoma cell line KB-3-1 using colchicine at concentrations up to 2 µg/ml [20].
Once an initial identity has been established in a cell lineage it must be stabilized.
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A mathematical model is established in a unit cell, containing two membranes with an air-gap in between and two neighboring channels, to study the coupled heat and mass transports in the QPMAHP.
We then, transfected pcDNA4/TO/myc-His where the full-length scapinin cDNA was inserted between the BamHI and EcoRI sites and established in a Hela cell line.
Demethylation mediated inducible expression of the CDH13, MAGEA1, MGMT, p73 and RASSF1A genes was established in an astrocytoma cell line (U251), demonstrating that expression of these genes is likely regulated by DNA methylation.
The toxicity of extracellular ATP is well established in a variety of cell types and was shown to be mediated by especially P2X7 activation in HeLa cells [ 35, 39- 45].
This has been established in a variety of cell lines undergoing apoptosis induced by pro-oxidant and non-oxidant pro-apoptotic agents in vitro.
Basal cell surface expression of TRAIL receptors, FAS and p75NTR, was established in a panel of ESFT cell lines.
Gene expression and pathway analysis have uncovered canonical signaling pathways that are well established in a specific type of cells.
Bivalency is not only resolved during differentiation but is also established in a step-wise manner in differentiated cell types.
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