As a result of established difficulties in assembling repetitive sequences11, we found repeat-rich sequences more frequently among the smaller T. parvula contigs (Fig. 1b).
Furthermore, due to (well established) difficulties in estimating cell dry weight in fiber slurries, biomass was not included in the recovery calculations for the SSCF/HYBRID experiments, i.e. part of the missing carbon will be biomass.
Based on Table 1, one would expect to find the same established or partially established difficulties identified in previous research in the responses from undergraduate students to the assessments.
In summary, our study yielded the following major findings: All established difficulties documented in our literature review (Table 1 ) were consistently found in responses from our own undergraduate biology students.
All established difficulties documented in our literature review (Table 1) were consistently found in responses from our own undergraduate biology students.
The examples of shamanism and Hindu and Daoist mysticism demonstrate the difficulty in establishing a single definition of mysticism.
Based on clinical features and confocal microscopic findings of the cornea, clinical diagnosis of filamentous fungal keratitis can be established with no difficulty in our patient.
The epidemiology and symptoms of HMPV infection have not been fully elucidated; one obstacle in establishing these data is the difficulty in establishing a laboratory diagnosis of the infection.
A spokesman for North Somerset Council said they were trying to establish if the difficulty in getting tickets online was a genuine problem or a deliberate ploy by Banksy.
North Somerset Council said it was trying to establish if the difficulty in getting tickets online was a real problem or a deliberate ploy by Banksy.
