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The kinetics parameters were established by optimization.
Two nested PCR detection methods were established by optimization of the annealing temperatures of primers.
For example, N depends on the composition of the flows of the interconnection streams that were obtained in the pre-design stage, established by optimization.
A rapid estimation method of the intracellular lipid content in microorganisms using a fluorescent probe, Nile red, was established by optimization of the Nile red staining and data processing.
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The figures of merit used for evaluation and comparison were acquired in the optimal conditions for each modifier, established by multivariate optimization of the main variables based on Doehlert designs.
In the method proposed in this work, the recovery fractions are established by an optimization problem in which the total number of theoretical stages of the equivalent configuration is minimized and the composition of the output streams are restricted in order to reach their desired value.
Here, ( {x}_l^{prime } ), ( {y}_l^{prime } ), and ( {z}_l^{prime } ) are the actual location coordinates of the virtual sampling point ( {S}_l^{prime } ) in the detection area, V l is the distance vector from the sampling point to the anchor node, and f x, f y, f z are the estimated values of the regression model established by the optimization model parameter.
Whether simply inhibiting MDM2 synthesis is most effectively achieved via targeting of splicing as described in the present report or through direct translational inhibition as previously found [ 20] remains to be established by further optimization and direct comparison although the data so far indicate comparable potency.
To test each probe for optimal efficacy, a second plate is designed for each target to enable the testing of various concentrations of each probe ranging from 25 nM to 225 nM in the presence of optimal primer concentrations (as already established by the primer-optimization plate in each case).
In this study, single objective optimization is established by Taguchi methodology and optimal factor settings for multi objective optimization of two output responses MRR and TWR collectively are identified using Grey relational analysis.
By applying low and high fidelity aerodynamic analysis tools, BWB aerodynamic design methodology is established by the combination of optimization design and inverse design methods.
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