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Electrical contact to each electrode was established by inserting a copper wire through the top end of the pipet barrel to make contact with the carbon layers.
The working length (WL) of each canal was established by inserting a #10 K-Flexofile into the root canal until the file tip became visible through the apical foramen under a stereomicroscope at 20× magnification, then subtracting 1 mm.
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Pneumoperitoneum was established by inserting an 18 G intravenous catheter into the abdominal right lower quadrant of the peritoneal cavity and insufflating the abdomen with CO2 to a pneumoperitoneal pressure of 12 mm Hg.
The ideal lower dentition was established by inserting a.018 × 0.025-in 0.025-in
dl-LacZ was established by inserting LacZ into the adenovirus genome E3 locus.
Luciferase constructs were established by inserting oligonucleotides containing the wild-type or mutant putative target site of the FKBP51 3′-UTR inthethe multi-cloning site of the psiCHECK2 vector (Promega, Fitchburg, WI, USA) (see Supporting Information Online, Supplementary Materials and Methods for more details).
The two initial mouse models for NF1 (Nf1 +/Fcr and Nf1 +/n31) were established more than 20 years ago, by inserting a neomycin cassette into the exonal region of Nf1 (Brannan et al., 1994; Jacks et al., 1994).
Continuous glucose monitoring was established 24 48 h before each study visit by inserting a single subcutaneous glucose sensor (SEVEN PLUS; Dexcom, Inc; San Diego, CA) into the subcutaneous tissue of the abdomen.
Test by inserting a thin knife blade.
Check the egg is cooked by inserting a skewer.
Test the cake is cooked by inserting a butter knife.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com