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The ideal lower dentition was established by inserting a.018 × 0.025-in 0.025-in
dl-LacZ was established by inserting LacZ into the adenovirus genome E3 locus.
Electrical contact to each electrode was established by inserting a copper wire through the top end of the pipet barrel to make contact with the carbon layers.
The working length (WL) of each canal was established by inserting a #10 K-Flexofile into the root canal until the file tip became visible through the apical foramen under a stereomicroscope at 20× magnification, then subtracting 1 mm.
Pneumoperitoneum was established by inserting an 18 G intravenous catheter into the abdominal right lower quadrant of the peritoneal cavity and insufflating the abdomen with CO2 to a pneumoperitoneal pressure of 12 mm Hg.
Luciferase constructs were established by inserting oligonucleotides containing the wild-type or mutant putative target site of the FKBP51 3′-UTR inthethe multi-cloning site of the psiCHECK2 vector (Promega, Fitchburg, WI, USA) (see Supporting Information Online, Supplementary Materials and Methods for more details).
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This is established first by inserting early dendrites into the body wall during development, followed by dendritic growth that is scaled to concurrent expansion of the larval body wall and receptive fields (Parrish et al., 2009).
By means of manipulation of electrode placement, i.e. repositioning, and the changes in electrical parameters, the users interact with the system and observe the resulting electrical field strength established by the inserted electrodes in real time.
Coculture systems were established by using transwell inserts (0.4 mm pore, polycarbonate membrane; Costar, Cambridge, MA, USA) and transferred to six-well culture plates.
The researchers also tested whether the blood progenitors could establish themselves in recipients by inserting them into mice.
(a), substituted "Court of Military Review" for "board of review" as name of reviewing body established by each Judge Advocate General, and inserted provisions setting out procedures for such Courts of Military Review, their composition and functions.
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