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Accuracy of the scheme is established by increasing the number of collocation points N until the solutions are consistent and further increases do not change the value of the solutions.
Since the Taylor cone formation is needed at the end of the needle tip in order to obtain NF, the minimum voltage required to form a Taylor cone was established by increasing the applied voltage from 15 at 1 kV intervals.
After PCR amplification, the melting curve was established by increasing the temperature from 55°C to 95°C.
In Denmark, rates of open-heart surgery increased by 70% and the median waiting times declined by half since 1994 when additional capacity for cardiac surgical care was established by increasing the number of operating theatres, equipment and personnel [30].
The high producing cell lines had been established by increasing the transgene copy number of the low producers in order to reduce effects caused by clonal variation (Maccani et al. 2014).
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The involvement of mitochondria was established by increased mitochondrial membrane potential and up and down regulation of Bcl2 and Bax proteins as well as HSP70.
Antemortem diagnosis is conventionally established by increased LVOT velocity reported by spectral Doppler echocardiogram studies and is augmented by the presence of supportive findings such as presence of a visible subvalvular ridge, left ventricular hypertrophy, post-stenotic aortic dilation and aortic insufficiency (O'Grady et al. 1989).
This shows that drug resistance may be established by simply increasing the concentration of one or more ligands, such as HGF (hepatocyte growth factor) and EGF, in a combinatorial fashion.
Since the discovery in 1988 that upon treatment with tumor necrosis factor (TNF), cells showed either features of apoptosis or a necrotic balloon-like morphology without nuclear disintegration, the concept that RCD can also occur via regulated necrosis has been established by an increasing number of studies ([ 3, 4] reviewed in [ 5]).
(Efficacy of the Tunicamycin in the induction of an unfolded protein response was first established by showing increased fluorescence in the strain SJ4005, which contains an integrated transgene of GFP under the control of the hsp-4 promoter).
SU11274 drug resistant cells were established by exposure to increasing concentrations of SU11274 (0.5 12 μM).
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