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A colony was established by breeding Cacna1fnob2 mice with in-house C57BL/6 mice at the University of Calgary Health Sciences Animal Resource Centre.
Gdap1 knockout (Gdap1−/−) mice were established by breeding Cre deleter mice with homozygous Gdap1flox/flox mice.
Transgenic lines were then established by breeding the 2 founders (F0) with non-transgenic females.
Cohorts of mice for phenotyping were established by breeding male Df11(1)/+ and Dp11(1)/+ mice with female ApoeKO/KO or ApcMin/+ mice (Fig S1A of Supporting Information).
CD22ΔE12 × BCR ABL double-Tg mice were established by breeding commercially obtained male BCR ABL (p190) Tg founder mice (B6.Cg-Tg (BCR/ABL) 623Hkp/J, Jackson Labs) with female CD22ΔE12-Tg CD22ΔE12-Tg et al., 2015).
CD22ΔE12xBCR-ABL CD22ΔE12xBCR-ABLere established by breeding commercially obtained mice BCR-ABL (p190) Tg founder mice (B6.Cg-Tg (BCR-ABL) 623Hkp/J, Jackson Labs) were festablishedE12-Tg mice.
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The YG8R mice were established by cross breeding YG8 human genomic YAC transgenic mice that contain the entire FXN gene and expanded GAA repeats, with heterozygous Fxn-knockout mice (12).
Knockout (Nrbp1 +/− ) and conditional (Nrbp1 +/flox ) Nrbp1 alleles were established in mice by breeding male chimeras with Cre and Flp deleters, respectively.
Trials involving cocoa, coffee and cola were established by the plant breeding, agronomy and soil science departments, and plant pathology staff were stationed at Nobsu for joint CRIS/CSD trials.
The value of this local parameter was estimated for 679 radiata pine families with differing levels of genetic improvement (as quantified by their GF Plus rating for wood density) that were growing in 18 trials established by the Radiata Pine Breeding Company.
Recently, HAB and LAB mouse lines have been established by selective and bidirectional breeding for high (HAB) and low (LAB) anxiety-related behavior measured on the elevated plus-maze (EPM [15], [16].
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